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KMID : 0359320000400010035
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Korean Journal of Veterinary Research
2000 Volume.40 No. 1 p.35 ~ p.41
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Quantitation of abamectin by HPTLC and its pharmacokinetics after intramuscular injection in pigs
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Abstract
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We established a new method to analyze abamectin using HPTLC (high performance thin layer chromatography) in order to obtain its pharmacokinetic profiles in pigs. Recovery of abamectin in pig serum after fluorescence derivatization was 80.01±3.82% at 0.1ppm anil 83.67±3.63% at 10ppm, respectively. Detection reproducibility in terms of coefficient variation (c.v.) was 3.09% and 2.74% (intra-day), and 3.71% and 51.7%(inter-day), for 0.1 and 10ppm, respectively. Pharmacokinetics of abamectin was studied in five Yorkshire-Landrace mixed bred male pigs (35.0±2.7㎏) administered intramuscularly 0.3㎎/㎏ b.w. Pharmacokinetic profiles of abamectin in pigs were described by the 1-compartment open model with first-order absorption and first-order elimination. AUC (area under the curve) was 262.65±16.44ng·day/㎖ and the biological elimination half-life (t_½, k_e) was 5.28±0.84 days, indicating somewhat high bioavailability and long half-life by the intramuscular route. We suggest intramucular injection of abamectin could be also used in place of the recommended route of its subcutaneous administration so fa.
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